Name

PlyG

Recommended Name ?N-acetylmuramoyl-L-alanine amidase
Systematic Name ?peptidoglycan amidohydrolase
Alternative Name ?endolysin PlyG
acetylmuramoyl-alanine amidase
acetylmuramyl-alanine amidase
acetylmuramyl-L-alanine amidase
N-acetylmuramic acid L-alanine amidase
N-acetylmuramyl-L-alanine amidase
N-acetylmuramylalanine amidase
N-acylmuramyl-L-alanine amidase
peptidoglycan amidohydrolase
Uniprot IDQ8LTE6
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?NAM amidase
Reaction ?1. Catalysis of the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides (R04112)
    EC: 3.5.1.28
    Corresponding Pfam domain: Amidase_2
    Evidence: predicted (PubMed: 12192412)
Source Organism ?Bacillus phage Gamma
Target Organism ?Bacillus anthracis
Bacillus cereus
Disease ?Anthrax (inhalation, cutaneous, intestinal) (Bacillus anthracis)
State ?Tested
Reference ?12192412


Studies found: 2

Antimicrobial Agent
Study Type
Model
Administration
Relevant ResultsAdverse Effects and Other IssuesReference ?
PlyG in vitro
  • diverse set of 14 Bacillus anthracis strains
  • set of 10 different Bacillus cereus strains
  • set of 3 Bacillus thuringiensis strains
 Efficient lytic activity against all 14 B. anthracis strains tested, including capsulated ones.

Only B. cereus strain RSVF1 was susceptible to lysin activity, within 20 sec 2 U of PlyG decreased titers by 17.000 fold, nearly sterilization occured at 2 min.

No lytic activity was observed with B. thuringiensis strains.		 12192412
PlyG in vivo
  • murine model of Bacillus anthracis infection
 intraperitoneal

 Single dose of lysin (50 U or 150 U in 0.5 mL) administered 15 min after inoculation with lethal dose of 10x6 B. cereus RSVF1 resulted in therapeutic effect 68.4% or 76.9% lysin-treated mice were rescued (depending on the dose of lysin). Moreover, prolonged survivals in remaining animals were noted. Other Issues
Higher dose of lysin resulted in approximatelly equivalent therapeutic effect, probably due to inability of enzyme reach the bacteria hidden within peritoneal folds.		 12192412