Name

PlyC

Recommended Name ?PlyC multimeric lysin
Alternative Name ?PlyC amidase
PlyC endolysin
Uniprot IDQ7Y3F1
Q7Y3F3
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?Other amidase/peptidase
Reaction ?1. Catalysis of the hydrolysis of the link between N-acetyl muramic acid and L-alanine in the streptococcal peptidoglycan
    EC: n/a
    Corresponding Pfam domain: CHAP
    Evidence: experimental (PubMed: 16818874)
Source Organism ?Streptococcus phage C1
Target Organism ?Streptococcus pyogenes
Note on TargetS.pyogenes is also known as Group A Streptococcus.PlyC also target Group C and E streptococci.
Disease ?Acute glomerulonephritis
Acute rheumatic fever
Impetigo
Infective endocarditis
Pharyngitis
Rheumatic heart disease
Scarlet fever
State ?Tested
Reference ?16818874
22807482


Studies found: 2

Antimicrobial Agent
Study Type
Model
Administration
Relevant ResultsAdverse Effects and Other IssuesReference ?
PlyC in vitro
  • more then 40 bacterial strains in a variety of species
  • set of ten different group A streptococcal strains
  • set of 8 different Lancefield groups of streptococci
  • set of representative oral streptococci
  • set of mixed Gram-positive and Gram-negative bacterial strains
Efficient lytic activity against every strain in set of group A streptococci. Within 5 min 100 U of PlyC sufficiently lysed bacterial cells decreasing viable streptococcal titers by an order of 3 logs in 5 sec; 4 logs in 1 min and 5 logs in 10 min.
Significant lytic activity against groups C and E streptococci (considerably less than that seen with group A strains).
No lytic activity against groups B, D, F, G, L, and N streptococci.

Very slow but reproducible lysis of all the Streptococcus gordonii strains tested, no activity against other oral streptococci.

No lytic activity against any of Gram-positive or Gram-negative bacterial strains.		 11259652
PlyC in vivo
  • murine model of oral colonization
 oral

 Single dose of PlyC (250 U) administered to mice prior to challenge with 10x7 streptococci resulted in efficient protection, reducing the occurence of mucosal colonization from 70.5% to 28.5% in lysin-treated mice after 24 h.
In another experiment single dose of lysin were sufficient to completely eliminate streptococci in 9 heavily colonized mice as early as 2 h post-treatment.		 Resistance
However, some animals were recolonized within 48 h probably due to presence of previous internalization of bacteria into mucous membrane. Despite the recolonization, isolated surviving streptococci were sensitive to PlyC and further experiments failed to identify any lysin-resistant colonies.		 11259652